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Electrochemical DNA Biosensor Based on Graphene and TiO 2 Nanorods Composite Film for the Detection of Transgenic Soybean Gene Sequence of MON89788
Author(s) -
Gao Hongwei,
Sun Min,
Lin Chao,
Wang Shubai
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200403
Subject(s) - differential pulse voltammetry , nanorod , biosensor , detection limit , electrode , electrochemistry , graphene , materials science , nanocomposite , intercalation (chemistry) , substrate (aquarium) , nuclear chemistry , dna , nanotechnology , chemistry , cyclic voltammetry , chromatography , inorganic chemistry , biochemistry , biology , ecology
Based on graphene (GR), TiO 2 nanorods, and chitosan (CTS) nanocomposite modified carbon ionic liquid electrode (CILE) as substrate electrode, a new electrochemical DNA biosensor was effectively fabricated for the detection of the transgenic soybean sequence of MON89788. By using methylene blue (MB) as hybridization indicator for monitoring the hybridization with different ssDNA sequences, the differential pulse voltammetric response of MB on DNA modified electrodes were recorded and compared. Due to the synergistic effects of TiO 2 nanorods and GR on the electrode surface, the electrochemical responses of MB were greatly increased. Under optimal conditions the differential pulse voltammetric response of the target ssDNA sequence could be detected in the range from 1.0×10 −12 to 1.0×10 −6 mol/L with a detection limit of 7.21×10 −13 mol/L (3 σ ). This electrochemical DNA biosensor was further applied to the polymerase chain reaction (PCR) product of transgenic soybeans with satisfactory results.