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Assessment of Cell Viability Using the Chronoamperometric Method Based on Screen‐Printed Electrodes
Author(s) -
Tsai Hweiyan,
Tsai Shangheng,
Deng Hweiwen,
Bor Fuh C.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200397
Subject(s) - ferricyanide , ferrocyanide , detection limit , electrochemistry , viability assay , electrode , chronoamperometry , linear range , menadione , materials science , potassium ferricyanide , chemistry , analytical chemistry (journal) , chromatography , cell , cyclic voltammetry , biochemistry , inorganic chemistry , enzyme
This study investigates suitable electrochemical mediators and optimal mediator concentration for breast cancer cells, MDA‐MB‐231. The optimized mediators were 1 mM ferricyanide and 10 µM menadione. The specifications for the chronoamperometric detection of ferrocyanide were a linear range of 0.02–0.36 mM. The detection limit was 5.0 µM and the spiked recovery was 95 %–99 %. The oxidation currents increased linearly with cultured cell density. Cell viabilities determined using electrochemical methods confirmed those determined by MTT assay. The running time can be reduced to less than 30 min. The chronoamperometric method can be used as an alternative method for rapidly assessing the viability of breast cancer cells.