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Design of an Amperometric Biosensor for the Determination of Biogenic Amines Using Screen Printed Carbon Working Electrodes
Author(s) -
Telsnig Dietlind,
Kalcher Kurt,
Leitner Andreas,
Ortner Astrid
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200378
Subject(s) - cadaverine , putrescine , tyramine , amperometry , biosensor , chemistry , hydrogen peroxide , flow injection analysis , chromatography , amine gas treating , biogenic amine , detection limit , potentiometric titration , electrode , organic chemistry , electrochemistry , biochemistry , receptor , neurotransmitter , enzyme
An amperometric biosensor with pea seedling amine oxidase and screen printed carbon electrode modified with MnO 2 for determining biogenic amines (BAs) was designed. The bio‐component was immobilized with Nafion solution. The enzymatically produced hydrogen peroxide was determined to quantify BAs. All measurements were performed in flow injection analysis system with phosphate buffer (66 mM, pH 7.5) as mobile phase at +400 mV vs. Ag/AgCl. A linear correlation was found for cadaverine and putrescine from 1–50 µM, for tyramine and histamine from 10–300 µM respectively. The limits of detection were determined to be 0.3 µM for cadaverine and putrescine and 3.0 µM for tyramine and histamine. The sensor was used to quantify BAs in chicken meat samples.