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Direct Electrochemistry of Heme Proteins on Electrodes Modified with Didodecyldimethyl Ammonium Bromide and Carbon Black
Author(s) -
Suprun Elena V.,
Arduini Fabiana,
Moscone Danila,
Palleschi Giuseppe,
Shumyantseva Victoria V.,
Archakov Alexander I.
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200359
Subject(s) - ammonium bromide , horseradish peroxidase , myoglobin , chemistry , electrochemistry , heme , electrode , bromide , electron transfer , hemeprotein , cytochrome c , carbon black , microelectrode , combinatorial chemistry , nuclear chemistry , inorganic chemistry , photochemistry , organic chemistry , enzyme , biochemistry , pulmonary surfactant , natural rubber , mitochondrion
A novel matrix based on commercially available carbon black (CB) N220 and didodecyldimethyl ammonium bromide (DDAB) was shown to be a reliable support for direct electron transfer reactions between screen printed electrode (SPE) and Fe(III)‐heme proteins. Cytochrome c (cyt c ), myoglobin (Mb), horseradish peroxidase (HRP) and cytochromes P450 (CYP 51A1, CYP 3A4, CYP 2B4) generated well‐shaped cyclic voltammograms on SPE/CB/DDAB electrodes (both in solution and in immobilized state). The attractive performance characteristics of CB modified electrodes are advantageous over single‐walled carbon nanotubes (SW CNT) based ones. The achieved direct electrochemistry of heme proteins on CB/DDAB‐modified electrodes provided successful elaboration of the immunosensor for cardiac Mb. The immunosensor showed applicability for diagnostics of myocardial infarction displaying significant difference in cardiac Mb content of human blood plasma samples taken from the corresponding patients.