Premium
Biomolecules Immobilization Using the Aryl Diazonium Electrografting
Author(s) -
Abdellaoui S.,
Corgier B. C.,
Mandon C. A.,
Doumèche B.,
Marquette C. A.,
Blum L. J
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200334
Subject(s) - biomolecule , biochip , biosensor , nanotechnology , combinatorial chemistry , aryl , surface modification , aniline , chemistry , oligonucleotide , macromolecule , materials science , organic chemistry , biochemistry , dna , alkyl
The electrografting of aryl‐diazonium was proven to be an efficient way to immobilize antibodies, oligonucleotides and enzymes onto conductive supports. Biomolecules chemically functionalized with aniline derivates or diazotated derivates could be used to build complex architectures acting as sensing layers for biosensors, biochips or others bioelectronic devices. Additionally, the use of SPR offers new opportunities to characterize the grafted surfaces as well as to develop label‐free assays. Here, we give an overview of our group achievements in the field during the last 7 years. We highlight the applications of these functionalized surfaces in multiparametric sandwich assays, in the label free detection and imaging of macromolecular interactions by SPRi and in the immobilization of dehydrogenase via its cofactor.