Premium
An Amperometric Immunosensor Based on a Gold Nanoparticle‐Diazonium Salt Modified Sensing Interface for the Detection of HbA1c in Human Blood
Author(s) -
Liu Guozhen,
Iyengar Sridhar G.,
Gooding J. Justin
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200333
Subject(s) - amperometry , colloidal gold , chemistry , ferrocene , nanoparticle , electrode , combinatorial chemistry , electrochemistry , chromatography , nuclear chemistry , materials science , nanotechnology
An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti‐HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti‐HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6 %–15.1 % of total hemoglobin in human blood by a competitive inhibition assay.