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A Simple Strategy to Improve the Accuracy of the Injection Step in Batch Injection Analysis Systems with Amperometric Detection
Author(s) -
Gimenes Denise Tofanello,
Pereira Polyana Fernandes,
Cunha Rafael Rodrigues,
Bezerra da Silva Rodrigo Amorim,
Munoz Rodrigo Alejandro Abarza,
Richter Eduardo Mathias
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200254
Subject(s) - analyte , amperometry , pipette , calibration curve , robustness (evolution) , computer science , calibration , chromatography , pulse (music) , biological system , chemistry , detection limit , analytical chemistry (journal) , electrode , mathematics , statistics , detector , telecommunications , biochemistry , biology , electrochemistry , gene
In this study, we describe for the first time the application of an internal standard method to compensate for random errors associated with the injection procedure in batch injection analysis (BIA) systems with multiple pulse amperometric detection. A sequence of potential pulses was selected in such a way that the internal standard (IS) compound was detected individually at one potential pulse and both the IS and analyte, were detected at another potential pulse. The current ratio ( I IS+analyte / I IS ) was used in the construction of the calibration curve and then to compensate for random errors. The use of disposable syringes or manual pipettes in BIA systems increases the robustness of the method and dispenses with skilled operators.