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Comparison of the Effects of Biofouling on Voltammetric and Potentiometric Measurements
Author(s) -
Kuhlmann Julia,
Dzugan Laura C.,
Heineman William R.
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200194
Subject(s) - biofouling , potentiometric titration , cyclic voltammetry , voltammetry , biomolecule , chemistry , biosensor , environmental chemistry , chromatography , electrode , electrochemistry , biochemistry , membrane
Biofouling of sensors is a common problem when measuring biological samples. The adherence of proteins and biomolecules, called hemostasis, is the first of four steps that lead to biofouling and eventually a foreign body response. This typically occurs within the first hours after the exposure of the biosensor to a biological sample. The purpose of this study was to assess the effect of this initial step of biofouling on cyclic voltammetry and potentiometric measurements. The results show that biofouling occurred rapidly within minutes and strongly affected cyclic voltammetry measurements, while potentiometric measurements were minimally affected even after 24 hours.