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A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine
Author(s) -
Li Shengxi,
Zhang Jiong,
Deng Wangping,
Shen Xizhong,
Song Shiping,
Fan Chunhai
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200166
Subject(s) - clenbuterol , detection limit , chromatography , amperometry , immunoassay , chemistry , bovine serum albumin , urine , biosensor , reproducibility , coefficient of variation , electrochemistry , electrode , antibody , biochemistry , medicine , immunology
We report a highly sensitive competitive‐type assay for multiplexing electrochemical detection of clenbuterol in pig urine using a 16‐sensor array. In this design, the clenbuterol was first conjugated with bovine serum albumin (BSA), and then the conjugates were immobilized on the electrode surface to compete with the free clenbuterol in the sample for specific antibody. Under the optimal conditions, the reproducibility of the clenbuterol electrochemical immunosensor was evaluated to be 3.4 % (coefficient of variation, CV) and the limit of detection was estimated to be 1.3 pg/mL. The very low detection limit was probably derived from the higher efficiency of the competitive immunoreaction caused by appropriate quantities of the clenbuterol immobilized on the 16‐sensor array and the suitable amount of anti‐clenbuterol antibody in the assay system.