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Capillary Electrophoresis Coupled with Electrochemiluminescence for the Facile Separation and Determination of Salbutamol and Clenbuterol in Urine
Author(s) -
Bao Yi,
Yang Fan,
Yang Xiurong
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200098
Subject(s) - clenbuterol , salbutamol , electrochemiluminescence , chromatography , capillary electrophoresis , urine , chemistry , detection limit , medicine , biochemistry , asthma
A capillary electrophoresis coupled with tris(2,2′‐bipyridyl) ruthenium(II) (Ru(bpy) 3 2+ ) electrochemiluminescence detection system was developed to determine salbutamol and clenbuterol in urine. Some factors that affected the performances of separation and detection were investigated. Under the optimized conditions, one single quantitative analysis of salbutamol and clenbuterol was achieved at a separation voltage of 15 kV within 9 min, and the LOD s ( S / N =3) and LOQ s ( S / N =10) of salbutamol and clenbuterol were 8.43×10 −8 mol/L, 2.61×10 −7 mol/L and 2.73×10 −7 mol/L, 8.21×10 −7 mol/L, respectively. The recovery obtained from the analysis of spiked urine samples was between 88.6 % and 104.7 % with RSD s lower than 6.70 %. The method was successfully applied to determine salbutamol and clenbuterol in urine samples.