Premium
Molecularly Imprinted Electrochemical Luminescence Sensor Based on Enzymatic Amplification for Ultratrace Isoproturon Determination
Author(s) -
Li Shuhuai,
Tao Huilin,
Li Jianping
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200088
Subject(s) - electrochemiluminescence , chemistry , detection limit , luminol , hydrogen peroxide , luminescence , electrochemistry , chemiluminescence , nuclear chemistry , chromatography , biochemistry , electrode , materials science , optoelectronics
A new molecularly imprinted electrochemical luminescence sensor (MIP‐ECL sensor) was developed for isoproturon (IPU) determination based on the competition reaction between IPU and glucose oxidase labeled IPU (GOD‐IPU). After competition, hydrogen peroxide produced by residual GOD‐IPU on the MIP reacted with luminol to emit electrochemiluminescence (ECL) signal. The ECL intensity decreased when the GOD‐IPU molecules were replaced by IPU molecules in the samples. IPU could be determined in the concentration range from 9×10 −11 mol/L to 5.1×10 −9 mol/L with a detection limit of 3.78×10 −12 mol/L. Water samples were assayed and recoveries ranging from 98.5 % to 102.1 % were obtained.