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Impedimetric Aptasensor for Thrombin Recognition Based on CD Support
Author(s) -
Castillo Gabriela,
Trnkova Libuse,
Hrdy Radim,
Hianik Tibor
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200041
Subject(s) - aptamer , thrombin , biosensor , detection limit , chemistry , combinatorial chemistry , electrochemistry , dielectric spectroscopy , ligand (biochemistry) , nanotechnology , redox , materials science , inorganic chemistry , electrode , chromatography , receptor , biochemistry , microbiology and biotechnology , platelet , immunology , biology
We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer‐protein formation at the surface of CD‐trodes (GCDTs) in the presence of the redox couple [Fe(CN) 6 ] 3−/4− . GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self‐assembling of a thiol‐modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 µM with a limit of detection of 5 nM.