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Electrochemical Evaluation of Binding Affinity for Aptamer Selection Using the Microarray Chip
Author(s) -
Zhu Ye,
Chandra Pranjal,
Ban Changill,
Shim YoonBo
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100734
Subject(s) - aptamer , dissociation constant , kanamycin , electrochemistry , chemistry , combinatorial chemistry , biosensor , nanotechnology , analytical chemistry (journal) , chromatography , materials science , electrode , microbiology and biotechnology , biochemistry , biology , receptor , gene
Simultaneous evaluation of the binding affinity of a series of aptamers toward a target molecule was investigated using an electrochemical microarray chip. The chip was modified by immobilizing seven aptamers obtained from the SELEX process and a control sequence onto gold nanoparticle‐comprised conducting polymer‐coated microarray electrodes. The chip was then incubated with the target molecule, kanamycin. The electrochemical response of the captured kanamycin was studied to evaluate the binding affinity of the aptamers, which showed the same trend with the fluorescence spectroscopic results. The lowest dissociation constant between a selected aptamer and kanamycin was determined to be 38.06±0.73 nM.