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Voltammetric Sensing of Amino Acids in the Presence of Cu(II) in Acidic and Alkaline Solutions
Author(s) -
Pauliukaite Rasa,
Pinto Edilson M.,
Amine Aziz,
Brett Christopher M. A.
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100733
Subject(s) - chemistry , alkaline hydrolysis , linear sweep voltammetry , cyclic voltammetry , electrolyte , inorganic chemistry , voltammetry , amino acid , electrochemistry , hydrolysis , copper , basic solution , decomposition , electrode , organic chemistry , biochemistry
A number of amino acids have been determined at carbon film electrodes in the presence of copper. Strongly acidic, 0.1 M HCl, in the presence of 0.1 mM Cu(II), as well as alkaline, 0.1 M NaOH, solution permit successful measurement of individual amino acids, clearer separation between oxidation of Cu and Cu‐complexes occurring in alkaline solution. Electrochemical impedance showed that Cu(II) facilitates charge transfer, particularly in alkaline medium. Square wave voltammetry with preconcentration increased the response compared to linear sweep voltammetry. Protein hydrolysis rates were monitored through determination of amino acids produced by decomposition, injecting samples into alkaline electrolyte solution.