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Electrochemical Oxidation Mechanisms of the Antioxidants Daidzein and 7‐Hydroxy‐4‐chromone
Author(s) -
Fernandes Isabel P. G.,
Oliveira Severino Carlos B.,
Ghalkhani Masoumeh,
Shahrokhian Saeed,
OliveiraBrett Ana Maria
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100629
Subject(s) - chromone , chemistry , electrochemistry , daidzein , ring (chemistry) , phosphate buffered saline , medicinal chemistry , diffusion , nuclear chemistry , stereochemistry , electrode , organic chemistry , chromatography , genistein , medicine , physics , thermodynamics
The electrochemical behavior of antioxidants daidzein (DZ) and 7‐hydroxy‐4‐chromone (7‐OH‐4‐CHM) was investigated. The oxidation of DZ is irreversible, pH‐dependent, and occurs in two steps, the first of 4′‐OH on the B‐ring and the second on the 7‐OH on the A‐ring. The oxidation of 7‐OH‐4‐CHM occurs on the 7‐OH on the A‐ring, is irreversible, pH‐dependent and proceeds in a single step. The DZ diffusion coefficient was calculated in pH 7.0 phosphate buffer to be D DZ =8.1×10 −5 cm 2 s −1 . The detection limits of DZ and 7‐OH‐4‐CHM was calculated as LOD DZ =0.08 µM and LOD 7‐OH‐4‐CHM =0.13 µM.