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Synergistic Effect of MWNTs/CeO 2 /CHIT Film for Detection of CdSe Nanoparticle Labeled Sequence‐Specific of 35S Promoter of Cauliflower Mosaic Virus Gene
Author(s) -
Yang Tao,
Huang Daming,
Li Qianhe,
Zhang Wei,
Jiao Kui
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100507
Subject(s) - cauliflower mosaic virus , gene , nanoparticle , sequence (biology) , gene sequence , virus , chemistry , virology , nanotechnology , microbiology and biotechnology , materials science , biology , biochemistry , transgene , genetically modified crops , phylogenetic tree
A porous composite film was fabricated combining the advantages of multiwalled carbon nanotubes, CeO 2 and chitosan. The synergistic effect of the film improved the immobilization of probe ssDNA. The loaded probe ssDNA was used for detection of CdSe quantum dots labeled target DNA. The DNA hybridization reaction was detected by differential pulse anodic stripping voltammetry of Cd 2+ after the oxidative release of labeled CdSe quantum dots. The established DNA biosensor can discriminate different target sequences associated with 35S promoter of cauliflower mosaic virus gene with relatively wide linear range and low detection limit (2.4×10 −13 mol/L).