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Development of an Integrated System for Immobilization and Mediating Charge to Alcohol Dehydrogenase During Bioelectrocatalytic Oxidation and Detection of Ethanol
Author(s) -
Kowalewska Barbara,
Kulesza Pawel J.
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100498
Subject(s) - alcohol dehydrogenase , amperometry , fourier transform infrared spectroscopy , biosensor , anode , chemical engineering , bioelectrochemistry , electrode , chemistry , substrate (aquarium) , ethanol , vinyl alcohol , materials science , nanotechnology , electrochemistry , organic chemistry , polymer , oceanography , geology , engineering
A robust biocatalytic electrode film utilizing multiwalled carbon nanotubes intentionally derivatized with poly(diallyldimethylammonium chloride), PDDA, as well as integrated with alcohol dehydrogenase is considered here for potential application as a stable efficient anode in a biofuel cell and a specific working electrode in amperometric sensors. PDDA‐modified CNTs were characterized using transmission electron microscopy (TEM) and infrared spectroscopy (FTIR). Once immobilized on a glassy carbon electrode substrate, they facilitate not only distribution of charge but also immobilization of alcohol dehydrogenase molecules. The resulting integrated bioelectrocatalytic system was able to induce oxidation of ethanol and NADH as well as to produce relatively high currents at a fairly low potential.