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A Novel Hydrogen Peroxide Sensor Based on the Direct Electron Transfer of Catalase Immobilized on Nano‐Sized NiO/MWCNTs Composite Film
Author(s) -
Shamsipur Mojtaba,
Asgari Mehdi,
Mousavi Mir Fazlollah,
Davarkhah Reza
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100453
Subject(s) - detection limit , hydrogen peroxide , amperometry , dielectric spectroscopy , electron transfer , biosensor , catalase , linear range , non blocking i/o , electrode , electrochemistry , materials science , composite number , chemistry , carbon nanotube , chemical engineering , analytical chemistry (journal) , nuclear chemistry , inorganic chemistry , nanotechnology , chromatography , catalysis , photochemistry , organic chemistry , composite material , enzyme , engineering
MWCNTs‐nanoNiO composite was used as a glassy carbon electrode modifier for construction of a novel catalase nanobiosensor for hydrogen peroxide. The immobilized catalase exhibited excellent electrocatalytic activity towards the reduction of H 2 O 2 . The resulting amperometric biosensor exhibited a linear response over a concentration range of 200 µM to 2.53 mM with a low detection limit of 19.0 µM. Electrochemical impedance measurements revealed that the modified electrode can be used for the sensitive detection of H 2 O 2 . The charge transfer resistance found to decrease significantly after enzymatic reaction of nanobiosensor with H 2 O 2 . The resulting impedance was highly sensitive to H 2 O 2 over a linear range of 19–170 nM with a detection limit of 2.4 nM.