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Analysis of Amperometric Spike Shapes to Release Vesicles
Author(s) -
Watson Daniel J.,
Gummi Rohit R.,
Papke Jason B.,
Harkins Amy B.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100441
Subject(s) - amperometry , spike (software development) , vesicle , depolarization , biophysics , chemistry , biological system , biology , computer science , biochemistry , electrode , electrochemistry , membrane , software engineering
Carbon‐fiber amperometry is a real‐time analytical tool to detect vesicle release events from individual secretory cells. This study compared the spike events released in response to local or global depolarization. We describe each spike shape that regularly occurs, as well as those shapes of spikes that occur with less frequency, but reproducibly. We compare how frequently each type of spike occurs, and compare their amplitudes and kinetics to the commonly accepted spike release events. Analysis of the amperometric peaks typically discarded from single cell amperometry recordings may reveal unappreciated phenomena related to the release of secretory vesicles.