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Development of a Catalase‐Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples
Author(s) -
Fűtő Péter,
Markus Gabriella,
Kiss Attila,
Adányi Nóra
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100399
Subject(s) - catalase , hydrogen peroxide , chemistry , biosensor , chromatography , amperometry , immobilized enzyme , sodium acetate , acetonitrile , enzyme , biochemistry , electrode , electrochemistry
The aim of our work was to develop a selective amperometric biosensor for infected milk detection. The catalase enzyme (EC 1.11.1.6) was immobilized onto the surface of a thin‐layer enzyme cell. The measurements were carried out in stopped‐flow injection analysis system (0.8 mL min −1 flow rate, +590 mV polarization potential) in organic phase (6 % sodium acetate buffer 200 mM pH 6.0, ferrocene conductive salt (7.5 mg L −1 ) dissolved in acetonitrile. A certain amount of hydrogen peroxide was degraded by the catalase content of the milk. The degradation of the hydrogen peroxide is proportional to the infection of the milk samples.