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Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy) 3 2+ ‐doped silica Nanoparticles as Signal‐Producing Compound
Author(s) -
Sun Qixia,
Zou Guizheng,
Zhang Xiaoli
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100331
Subject(s) - electrochemiluminescence , detection limit , electrode , dna , logarithm , nanoparticle , analytical chemistry (journal) , materials science , signal (programming language) , chemistry , nanotechnology , chromatography , biochemistry , computer science , programming language , mathematical analysis , mathematics
Abstract An electrochemiluminescence (ECL) DNA sensor was presented using a hairpin structure DNA as recognition element and Ru(bpy) 3 2+ ‐doped silica nanoparticles (Ru‐DSNPs) as ECL signal‐producing compound. The sensor was fabricated by immobilizing a hairpin structure DNA probe labeled with Ru‐DSNPs onto a gold electrode and formimg the stem‐loop structure. This sensor generated a strong ECL. After hybridization with the target DNA, the ECL signal significantly decreased. The logarithm of ECL intensity decreased linearly with the logarithm of target DNA concentration in the range from 2.0×10 −13 to 2.0×10 −9 mol/L with detection limit was 5.0×10 −14 mol/L ( S / N =3).