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Contactless Conductivity Detection in Capillary Electrophoresis Employing Capillaries with Very Low Inner Diameters
Author(s) -
Tůma Petr,
Samcová Eva,
Štulík Karel
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100264
Subject(s) - conductivity , capillary electrophoresis , thermal conductivity detector , capillary action , electrolyte , analytical chemistry (journal) , chemistry , electrophoresis , detection limit , detector , signal (programming language) , chromatography , materials science , electrode , optics , physics , composite material , gas chromatography , computer science , programming language
The behaviour of a contactless conductivity detector was studied in its application to capillary electrophoresis employing quartz capillaries with inner diameters ( i.d .) of 10 to 75 µm. The detector output signal was measured using KCl as the test electrolyte, within a KCl concentration range from 0 to 100 mmol/L, corresponding to specific conductivities from 0 to 1295 mS/m. When using capillaries with high inner diameters, then the signal‐to‐noise ratio is high in electrolytes of low conductivity. On the other hand, the use of capillaries with low i.d . values is useful in separations employing solutions of high conductivities. The advantages of capillaries with small i.d . values, combined with contactless conductivity detection, are demonstrated on separations of mixtures of inorganic ions and on separations of neutral mono‐ and disaccharides.