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Amperometric Immunosensor for Chagas’ Disease Using Gold CD‐R Transducer
Author(s) -
Vinicius Foguel Marcos,
Pilon dos Santos Glauco,
Aparecido Pupim Ferreira Antonio,
Yamanaka Hideko,
Vicente Benedetti Assis
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100260
Subject(s) - chagas disease , amperometry , trypanosoma cruzi , chromatography , antibody , gold standard (test) , chemistry , electrode , virology , medicine , immunology , electrochemistry , parasite hosting , world wide web , computer science
Chagas’ disease has been spread mainly through blood transfusion or blood components, therefore the development of a device for neglected diseases with high specificity and sensitivity is important to public health. This work describes the development of an amperometric immunosensor for determination of Chagas’ disease through a gold‐based electrode obtained from a recordable compact disc modified with 4‐(methylmercapto)benzaldehyde for the immobilization of Tc85 protein of the Trypanosoma cruzi . The immunoassays were carried out using positive and negative sera from Chagas’disease patients and immunoglobulin conjugated with peroxidase enzyme. The immunosensor presented −0.949 µA as cut‐off value and was applied in sera samples.