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Preparation of an Electrode Modified with an Electropolymerized Film as a Mediator of NADH Oxidation and Its Application in an Ethanol/O 2 Biofuel Cell
Author(s) -
Masuda Miyuki,
Motoyama Yusuke,
Murata Kenichi,
Nakamura Nobuhumi,
Ohno Hiroyuki
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100215
Subject(s) - bilirubin oxidase , nad+ kinase , alcohol dehydrogenase , electrochemistry , chemistry , nicotinamide adenine dinucleotide , anode , ethanol , electrocatalyst , electrode , catalysis , inorganic chemistry , nuclear chemistry , organic chemistry , enzyme
This paper reports a novel mediator for the oxidation of β ‐nicotinamide adenine dinucleotide (NAD + /NADH), an electropolymeric film (pAPRu) of [Ru(NH 2 ‐phen) 3 ] 2+ . A pAPRu‐modified electrode was prepared via electropolymerization and exhibited catalytic activity toward the electrochemical oxidation of NADH due to the imine moieties of pAPRu. The electrochemical oxidation of ethanol was observed using an alcohol dehydrogenase (ADH)‐immobilized electrode. A compartmentless ethanol/O 2 biofuel cell composed of an ADH anode and a bilirubin oxidase cathode was constructed. The maximum current density and the maximum power density of the biofuel cell were 190 µA cm −2 and 31 µW cm −2 (at 0.29 V), respectively.