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A Fast and Direct Amperometric Determination of Hg 2+ by a Bienzyme Electrode Based on the Competitive Activities of Glucose Oxidase and Laccase
Author(s) -
Cosnier Serge,
Mousty Christine,
Guelorget Amandine,
Lopez Marta SanchezPaniagua,
Shan. Dan
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100200
Subject(s) - amperometry , biosensor , glucose oxidase , laccase , chemistry , electrode , chromatography , abts , immobilized enzyme , nuclear chemistry , inorganic chemistry , enzyme , electrochemistry , biochemistry , antioxidant , dpph
A new concept of enzyme inhibition‐based biosensor involving the appearance of an amperometric signal for an inhibition by mercury was developed. The bienzyme sensor was composed of two layers of clay materials. The inner layer was constituted of layered double hydroxides entrapping laccase wired by ABTS. The outer laponite layer contained glucose oxidase (GOD). GOD catalyzed the glucose oxidation with the reduction of O 2 into H 2 O 2 . This induced a drastic decrease of the biosensor response to O 2 by the electrically wired laccase. HgCl 2 inhibited the O 2 consumption by GOD leading to a signal increase of the electroenzymatic reduction of O 2 .