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A Biosensor Based on Polyaniline‐Carbon Nanotube Core‐Shell for Electrochemical Detection of Pesticides
Author(s) -
Cesarino Ivana,
Moraes Fernando C.,
Machado Sergio A. S.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100161
Subject(s) - chronoamperometry , cyclic voltammetry , detection limit , polyaniline , dielectric spectroscopy , carbon nanotube , fourier transform infrared spectroscopy , biosensor , materials science , analytical chemistry (journal) , nuclear chemistry , chemistry , chemical engineering , electrochemistry , electrode , nanotechnology , chromatography , polymer , polymerization , composite material , engineering
A core‐shell structure based on polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT) films was electrodeposited on glassy carbon (GC) for the immobilization of acetylcholinesterase (AChE) as enzyme, in developing a biosensor for pesticides. The characterization and advantages of using the hybrid materials were examined by Fourier transform infrared spectroscopy (FTIR), field‐emission gun scanning electron microscopy (FEG‐SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The immobilization of AChE on the surface of the electrode was largely improved due to the unique synergistic effect of PANI and MWCNT. The developed GC/MWCNT/PANI/AChE biosensor was evaluated for determination of carbamate by square wave voltammetry (SWV) and chronoamperometry. SWV allowed carbaryl to be determined in a linear range between 9.9 and 49.6 nmol L −1 , with a detection limit of 4.6 nmol L −1 and using chronoamperometry the analytical curve was linear in the concentration ranges from 9.9 to 49.6 µmol L −1 , with a detection limit of 1.4 µmol L −1 .