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Immobilization of Xanthine Oxidase on Carbon Nanotubes Through Double Supramolecular Junctions for Biosensor Construction
Author(s) -
Villalonga Reynaldo,
Diez Paula,
Gamella María,
Reviejo Julio,
Pingarrón José M.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100153
Subject(s) - adamantane , biosensor , carbon nanotube , supramolecular chemistry , surface modification , stacking , synthon , pyrene , chemistry , xanthine oxidase , nanotube , combinatorial chemistry , nanotechnology , materials science , organic chemistry , enzyme , molecule
A novel approach for the noncovalent functionalization of single‐walled carbon nanotubes with enzymes, using a β‐cyclodextrin‐modified pyrene derivative, mono‐6‐ethylenediamino‐(2‐pyrene carboxamido)‐6‐deoxy‐β‐cyclodextrin (Pyr‐βCD), as a molecular bridge for the construction of a supramolecular assembly between the nanotube surface and an adamantane‐modified enzyme, is reported. The Pyr‐βCD derivative was synthesized and its stacking to SWNT through π–π interactions accomplished. The functionalized nanotubes showed low capacity for the nonspecific adsorption of proteins, but were able to immobilize adamantane‐modified xanthine oxidase via host‐guest associations. This double supramolecular junctions‐based approach was employed to modify a glassy carbon electrode with the enzyme/nanotubes complex for designing a biosensor device toward xanthine. The biosensor showed fast electroanalytical response (10 s), high sensitivity (5.9 mA/M cm 2 ) low detection limit (2 µM) and high stability.