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Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs
Author(s) -
Bartošík Martin,
Paleček Emil
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100079
Subject(s) - electrode , hanging mercury drop electrode , guanine , voltammetry , square wave , analytical chemistry (journal) , electrochemistry , chemistry , amalgam (chemistry) , anodic stripping voltammetry , dna , carbon paste electrode , materials science , cyclic voltammetry , chromatography , voltage , biochemistry , physics , nucleotide , quantum mechanics , gene
We show that, in difference to previously applied electrochemical methods working with stationary electrodes, square wave voltammetry produces well‐developed peaks II SW (specific for dsDNA) and III SW yielded by ssDNA at hanging mercury drop electrode (HMDE) and solid amalgam electrodes (SAEs). Using these peaks various kinds of DNA structural transitions can be studied, including unwinding of dsDNA at negatively charged electrode surfaces. The sensitivity of the DNA analysis is much better than that obtained with guanine oxidation signals at carbon electrodes. Both carbon electrodes and SAEs appear attractive as transducers in label‐free RNA and DNA sensors.