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A New Approach how to Define the Coefficient of Electroactivity of Adenine and Its Twelve Derivatives Using Flow Injection Analysis with Amperometric Detection
Author(s) -
Zitka Ondrej,
Skutkova Helena,
Adam Vojtech,
Trnkova Libuse,
Babula Petr,
Hubalek Jaromir,
Provaznik Ivo,
Kizek Rene
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100064
Subject(s) - detection limit , adenosine , flow injection analysis , chemistry , adenosine monophosphate , nicotinamide adenine dinucleotide , electrochemistry , amperometry , electrode , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , enzyme , nad+ kinase
We studied the electrochemical behaviour of adenine derivates (adenosine, 2‐aminopurine, 2,6‐diaminopurine, 6‐benzyl‐aminopurine, adenosine monophosphate, cyclic adenosine monophosphate, nicotinamide adenine dinucleotide, adenosine triphosphate, S ‐adenosyl‐ L ‐methionine, and synthetic derivatives AD‐3, AD‐6 and AD‐9) using flow injection analysis with electrochemical detection using a glassy carbon electrode. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mL min −1 and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters. The lowest detection limit was estimated for adenine as 0.9 nM and 2‐aminopurine as 0.5 nM.