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Sensitive and Selective Detection of Dopamine Using a DNA Immobilized Ethylenedidamine/Polyglutamic Modified Electrode
Author(s) -
Zou Lina,
Luo Peili,
Li Yamin,
Zhang Shusheng,
Ye Baoxian
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000724
Subject(s) - detection limit , ascorbic acid , electrode , polyglutamic acid , dopamine , chemistry , uric acid , linear range , chromatography , dna , nuclear chemistry , biochemistry , medicine , endocrinology , food science
DNA was attached on the surface of an ethylenedidamine/polyglutamic(En/PGA) modified glassy carbon electrode (GCE) to create a novel voltammetric sensor (DNA/En/PGA/GCE) for dopamine (DA). This modified electrode exhibited a linear voltammetric response for DA in the range from 1.0×10 −7 mol L −1 to 1×10 −5 mol L −1 , with a detection limit of 2×10 −8 mol L −1 . The detection of DA was found to be unaffected by the presence of ascorbic acid, uric acid, serotonin and folic acid. The method proposed was applied to detect DA in pharmaceutical dosage and human blood serum with good satisfactory results.