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Determinatiom of 8‐Hydroxy 2′‐Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
Author(s) -
Gutiérrez Alejandro,
Gutiérrez Silvia,
García Guadalupe,
Galicia Laura,
Rivas Gustavo A.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000677
Subject(s) - polyethylenimine , ascorbic acid , detection limit , carbon nanotube , differential pulse voltammetry , chemistry , biomolecule , 8 hydroxy 2' deoxyguanosine , voltammetry , electrochemistry , electrode , inorganic chemistry , cyclic voltammetry , nuclear chemistry , dna , chromatography , materials science , dna oxidation , nanotechnology , dna damage , biochemistry , transfection , food science , gene
Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8‐hydroxy 2′‐deoxyguanosine (8‐OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8‐OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8‐OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0×10 −7 M to 3.0×10 −5 M, with detection limit ( DL ) of 1.0×10 −7 M and the quantification limit ( QL ) of 3.0×10 −7 M.