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Enzyme Logic Gates Based on Enzyme‐Coated Carbon Nanotubes
Author(s) -
Kim KyungWoo,
Kim Byung Chan,
Lee Hye Jin,
Kim Jungbae,
Oh MinKyu
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000634
Subject(s) - glucose oxidase , horseradish peroxidase , enzyme , carbon nanotube , chemistry , covalent bond , invertase , signal (programming language) , materials science , nanotechnology , biochemistry , computer science , organic chemistry , programming language
Enzyme‐based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three‐step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC‐GOx and EPC‐INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly‐usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems.