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A Solid‐State Electrochemiluminescence Immunosensor Based on MWCNTs‐Nafion and Ru(bpy) 3 2+ /Nano‐Pt Nanocomposites for Detection of α ‐Fetoprotein
Author(s) -
Cao Yaling,
Yuan Ruo,
Chai Yaqin,
Mao Li,
Yang Xia,
Yuan Shirong,
Yuan Yali,
Liao Yuhong
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000611
Subject(s) - electrochemiluminescence , detection limit , nafion , carbon nanotube , nanocomposite , materials science , electrode , nanoparticle , selectivity , linear range , nanotechnology , analytical chemistry (journal) , chemistry , catalysis , chromatography , electrochemistry , organic chemistry
A approach was successfully employed for constructing a solid‐state electrochemiluminescence (ECL) immunosensor by layer‐by‐layer self‐assembly of multiwall carbon nanotubes (MWCNTs)‐Nafion composite film, Ru(bpy) 3 2+ /nano‐Pt aggregates (Ru‐PtNPs) and Pt nanoparticles (PtNPs). The influence of Pt nanoparticles on the ECL intensity was quantitatively evaluated by calculating the electroactive surface area of different electrodes with or without PtNPs to immobilize Ru(bpy) 3 2+ . The principle of ECL detection for target α ‐fetoprotein antigen (AFP) was based on the increment of resistance after immunoreaction, which led to a decrease in ECL intensity. The linear response range was 0.01–10 ng mL −1 with the detection limit of 3.3 pg mL −1 . The immunosensor exhibited advantages of simple preparation and operation, high sensitivity and good selectivity.