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Determination of Ofloxacin in Pharmaceuticals, Human Urine and Serum Using a Potentiometric Sensor
Author(s) -
Pimenta A. M.,
Souto M. R. S.,
Catarino R. I. L.,
Leal M. F. C.,
Lima J. L. F. C.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000608
Subject(s) - ofloxacin , potentiometric titration , urine , chromatography , chemistry , pharmacology , medicine , electrode , biochemistry , antibiotics , ciprofloxacin
An ofloxacin‐selective electrode was developed for application in pharmaceutical preparations, serum and urine analysis. The electrode is based on tetrakis[3,5‐bis(trifluoromethyl)phenyl]borate as ion exchanger in a poly(vinyl chloride) membrane. The sensor displayed a Nernstian response for ofloxacin over a wide concentration range (3×10 −6 –1×10 −2 mol L −1 ) with a slope of 55±1 mV per decade of concentration and a practical detection limit of 1×10 −6 mol L −1 . The electrode was successfully applied to the determination of ofloxacin in samples with no pretreatment steps, adequate accuracy and precision and recoveries within the intervals of 94–107 %.