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Aptamer‐Based Inhibition Assay for the Electrochemical Detection of Tobramycin Using Magnetic Microparticles
Author(s) -
GonzálezFernández Eva,
delosSantosÁlvarez Noemí,
LoboCastañón María Jesús,
MirandaOrdieres Arturo J.,
TuñónBlanco Paulino
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000567
Subject(s) - aptamer , tobramycin , biotinylation , conjugate , chemistry , analyte , chromatography , selectivity , reproducibility , nanotechnology , materials science , antibiotics , biochemistry , microbiology and biotechnology , mathematical analysis , mathematics , gentamicin , catalysis , biology
The detection of small molecules using aptamers is not straightforward when the induced fit triggered by the analyte is also small. Herein a labeling strategy is explored for the voltammetric detection of the antibiotic tobramycin using magnetic microparticles (MPs). A competition between MPs‐immobilized tobramycin and free tobramycin for the limited amount of the biotinylated‐aptamer was established. The addition of the enzymatic conjugate and the substrate generated a product that was electrochemically oxidized on screen‐printed carbon electrodes. The analytical signal was proportional to the antibiotic concentration from 5–500 µM and the reproducibility was 5.7 %. The assay selectivity was also studied.