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Amperometric Tyrosinase Biosensor Based on Carbon Nanotube‐Doped Sol‐Gel‐Derived Zinc Oxide–Nafion Composite Films
Author(s) -
Lee Joong Min,
Xu GuangRi,
Kim Byung Kun,
Choi Han Nim,
Lee WonYong
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000556
Subject(s) - biosensor , nafion , amperometry , carbon nanotube , materials science , tyrosinase , detection limit , mesoporous material , nanocomposite , chemical engineering , composite number , phenol , electrode , nanotechnology , chromatography , chemistry , electrochemistry , composite material , organic chemistry , catalysis , engineering , enzyme
A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)–ZnO–Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17 nm) of the composite film, the biosensor exhibits very fast response time of 2 s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10 −8 M ( S/N =3) for phenol. The biosensor retains 88 % of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.