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Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode
Author(s) -
Yardım Yavuz,
Erez M. Emre
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000551
Subject(s) - electroanalytical method , electrode , indole 3 acetic acid , detection limit , cyclic voltammetry , electrochemistry , chemistry , voltammetry , analytical chemistry (journal) , boron , inorganic chemistry , chromatography , organic chemistry , potentiometric titration , auxin , biochemistry , gene
In this work, a boron‐doped diamond (BDD) electrode was used for the electroanalytical determination of indole‐3‐acetic acid (IAA) phytohormone by square‐wave voltammetry. IAA yielded a well‐defined voltammetric response at +0.93 V (vs. Ag/AgCl) in Britton–Robinson buffer, pH 2.0. The process could be used to determine IAA in the concentration range of 5.0 to 50.0 µM ( n =8, r =0.997), with a detection limit of 1.22 µM. The relative standard deviation of ten measurements was 2.09 % for 20.0 µM IAA. As an example, the practical applicability of BDD electrode was tested with the measurement of IAA in some plant seeds.