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Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA
Author(s) -
Ahmadi Farhad,
Jafari Batool
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000516
Subject(s) - fenitrothion , chemistry , cyclic voltammetry , voltammetry , analytical chemistry (journal) , dna , nuclear chemistry , electrochemistry , chromatography , biochemistry , electrode , pesticide , agronomy , biology
The interaction of Fenitrothion with DNA has been studied using the voltammetric and spectroscopic methods. An irreversible reduction was concluded from the CV data of Fenitrothion. The values of ( αn ) f =1.91 and ( αn ) b =2.06 were measured and the reduction process of Fenitrothion was performed with 4 electrons. By using non‐linear regression analysis of CV data, the binding constant, binding site size, and diffusion coefficient for free Fenitrothion ( D f ) and DNA‐Fenitrothion ( D b ) were calculated as: 1.03×10 4 , 1.204, 5.2×10 −4 and 1.72×10 −5 , respectively. Also the voltammetric, spectroscopic, thermodynamic and activation energy of DNA‐Fenitrothion complex revealed that the mode of interaction might be partial‐intercalative and complex formation is entropy favored.