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Simultaneous Determination of Acetaminophen (Paracetamol) and Ascorbic Acid in Pharmaceutical Formulations by LC Coupled to a Screen Printed Carbon Based Amperometric Detector
Author(s) -
Sarakbi Ahmad,
Aydogmus Zeynep,
Sidali Tarik,
Gokce Gültekin,
Kauffmann JeanMichel
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000494
Subject(s) - detection limit , amperometry , ascorbic acid , chromatography , nafion , chemistry , analyte , electrode , electrochemistry , food science
The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at +0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP‐C18 and a monolithic RP‐C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer‐methanol 91 : 9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2×10 −7 –1×10 −4 M. Limit of detection ( LOD ) for APAP and AA was 2×10 −7 and 5×10 −7 M, respectively and the limit of quantification ( LOQ ) was 5. 10 −7 and 1×10 −6 M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations.