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A Sensitive Glucose Biosensor Based on Brushite, a Biocompatible Cement
Author(s) -
López M. SánchezPaniagua,
LópezRuiz B.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000489
Subject(s) - biosensor , brushite , nafion , glucose oxidase , ascorbic acid , detection limit , chemistry , immobilized enzyme , chromatography , uric acid , materials science , electrode , nuclear chemistry , biochemistry , organic chemistry , electrochemistry , enzyme , calcium , food science
A highly sensitive biosensor based on glucose oxidase (GOx) immobilized on brushite cement is described. Biosensor was kept free of the interferences produced by ascorbic and uric acid by placing an extra Nafion film on the surface of the platinum electrode. Variables involved in the enzyme immobilization method were investigated. The biosensor exhibits a rapid response (10 s); a wide linear range (9×10 −6 M to 3×10 −3 M); low detection limit (9 µM); and high sensitivity (52.5±0.6 mA M −1 cm −2 ). Nafion/GOx/brushite biosensor was used to monitor the glucose levels of serum samples and satisfactory results were obtained.