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A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection
Author(s) -
Zani Angela,
Laschi Serena,
Mascini Marco,
Marrazza Giovanna
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000486
Subject(s) - multiplexing , microfluidics , biosensor , microfluidic chip , nanotechnology , lab on a chip , materials science , computer science , immunoassay , transducer , chip , chromatography , chemistry , antibody , engineering , electrical engineering , telecommunications , immunology , biology
In this paper, two simple and sensitive approaches for prostate specific antigen (PSA) detection are presented, by using antibody modified paramagnetic microparticles coupled to multiplexed electrochemical platforms. The first proposed approach is based on 8‐sensors screen‐printed arrays as candidate for electrochemical transducers and a simple target capturing step by means of antibody‐functionalised magnetic beads. In order to improve the performances of the immunosensing, a second approach is developed by using GRAVI‐Cell. This is microfluidic‐based affinity assay device for running bead‐protocols. This innovative system combines a special chip containing eight polymer microchannels, with a portable, computer‐controlled instrument. Both immunosensing strategies developed promise to be a sensitive, multiplexed tool for fast and easy PSA analysis.