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Changes in Performance of DNA Biosensor Caused by Hydroxyl Radicals
Author(s) -
Nowicka Anna M.,
Kowalczyk Agata,
Scholz Fritz,
Stojek Zbigniew
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000430
Subject(s) - methylene blue , radical , biosensor , chemistry , electrochemistry , layer (electronics) , dna , methylene , photochemistry , analytical chemistry (journal) , electrode , chromatography , organic chemistry , biochemistry , photocatalysis , catalysis
A DNA biosensor was exposed to the activity of OH radicals at two selected stages of its preparation/performance. The sensor was immersed in Fenton solution after immobilization of probe ssDNA layer and after the hybridization process with target DNA strands. It appeared that the extent of damage to ssDNA at the sensing layer was much stronger compared to the dsDNA layer. The visualization of the changes in the performance of the sensor caused by the action of OH radicals was done by applying methylene blue as an electrochemical tag. To explain the changes of the voltammetric peak of methylene blue for two selected stages of the sensor preparation the EIS measurements were also performed.