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Electrochemical Monitoring of Nucleic Acid Hybridization by Single‐Use Graphene Oxide‐Based Sensor
Author(s) -
Muti Mihrican,
Sharma Surbhi,
Erdem Arzum,
Papakonstantinou Pagona
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000425
Subject(s) - differential pulse voltammetry , dielectric spectroscopy , graphene , nucleic acid , dna–dna hybridization , chemistry , electrochemistry , combinatorial chemistry , electrode , nucleic acid thermodynamics , dna , oxide , hybridization probe , cyclic voltammetry , nanotechnology , materials science , biochemistry , base sequence , organic chemistry
In this paper, the application of a Graphene oxide (GRPox) integrated on single‐use pencil graphite electrode (PGE) for the enhanced monitoring of nucleic acids and for the sensitive and selective detection of the label free DNA hybridization related to Hepatitis B virus (HBV) sequences is described. Electrochemical behaviors of GRPox modified PGEs were firstly investigated using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined voltammetrically in the case of hybridization between amino linked probe and its complementary (target), or, noncomplementary (NC), or target/mismatch (MM) mixtures (1 : 1).