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An Electrochemical Immunosensor for Testosterone Using Gold Nanoparticles – Carbon Nanotubes Composite Electrodes
Author(s) -
Serafín V.,
Eguílaz M.,
Agüí L.,
YáñezSedeño P.,
Pingarrón J. M.
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000419
Subject(s) - colloidal gold , amperometry , carbon nanotube , electrochemistry , biomolecule , catechol , nanoparticle , electrode , composite number , chemistry , redox , nuclear chemistry , materials science , nanotechnology , inorganic chemistry , organic chemistry , composite material
A novel electrochemical immunosensor for testosterone based on direct attachment of antitestosterone on hybrid gold nanoparticles/carbon nanotubes‐Teflon composite electrodes is reported. This material combined the ability of gold nanoparticles for biomolecules immobilization retaining their biological activity with synergic electrocatalytic effect demonstrated by coupling gold nanoparticles and carbon nanotubes. Competitive assay between testosterone and HRP‐ testosterone for binding sites of antibodies was employed. Amperometry at −0.05 V vs. Ag/AgCl was used to monitor affinity reaction upon addition of H 2 O 2 with catechol as redox mediator. Calibration plot was linear between 0.1 and 10 ng/mL testosterone. LOD value was 85±6 pg/mL. Good results were obtained in the application to spiked human serum.