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An Amperometric Immunosensor for IgG Based on Conducting Polymer and Carbon Nanotube‐Linked Hydrazine Label
Author(s) -
Zhu Ye,
Koh Wei Choon Alvin,
Shim YoonBo
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000394
Subject(s) - amperometry , detection limit , carbon nanotube , hydrazine (antidepressant) , hydrogen peroxide , nuclear chemistry , conjugate , polymer , linear range , chemistry , electrode , covalent bond , materials science , electrochemistry , chromatography , nanotechnology , organic chemistry , mathematical analysis , mathematics
An amperometric immunosensor for IgG was developed by covalently immobilizing anti‐IgG on multiwall carbon nanotube‐embedded conducting polymer, poly‐5,2′ : 5′′,2′′‐terthiophene‐3′‐carboxylic acid (MWCNT/pTTCA). The MWCNT/pTTCA modified electrode was characterized by SEM, EIS, and XPS. A hydrazine‐labeled secondary antibody‐MWCNT conjugate (Hyd‐MWCNT‐Ab 2 ) was applied for detection. Hydrazine was used as a catalyst for the reduction of hydrogen peroxide, which was monitored at −0.3 V vs. Ag/AgCl. The calibration plots showed a linear range of 0.1–10 ng/mL with a detection limit of 0.084±0.004 ng/mL. The proposed immunosensor was evaluated for clinical applications in a rabbit serum sample.
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