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Simultaneous Differential Pulse Adsorptive Stripping Determination of Imipramine and Its Metabolite Desipramine by the PLS‐1 Multivariate Method
Author(s) -
GaleanoDíaz Teresa,
AcedoValenzuela MaríaIsabel,
MoraDiez Nielene,
SilvaRodríguez Antonio
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000371
Subject(s) - desipramine , metabolite , analyte , chromatography , chemistry , stripping (fiber) , adsorptive stripping voltammetry , differential pulse voltammetry , voltammetry , pulse (music) , glassy carbon , analytical chemistry (journal) , electrode , materials science , cyclic voltammetry , electrochemistry , biochemistry , antidepressant , neuroscience , detector , hippocampus , composite material , biology , electrical engineering , engineering
A method, using stripping differential pulse voltammetry, for the simultaneous determination of Imipramine and its metabolite Desipramine is reported. Both compounds produce, at glassy carbon electrode, an electrochemical signal due to an adsorptive‐oxidative process. The voltammograms show a very high overlap degree between IM and DE peaks. The multivariate calibration method PLS‐1 was employed for the simultaneous determination of both compounds. An experimental design together with the response surface methodology has been used to find the optimum experimental conditions. The developed procedure has been utilized in the analysis of fortified human serum samples with good recovery values for each analyte.