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Electrochemical DNA Sensing at Single‐walled Carbon Nanotubes Chemically Assembled on Gold Surfaces
Author(s) -
SantiagoRodríguez Lenibel,
SánchezPomales Germarie,
Cabrera Carlos R.
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000305
Subject(s) - carbon nanotube , electrochemistry , guanine , dna , monolayer , methylene blue , dna–dna hybridization , detection limit , materials science , hybridization probe , biosensor , nucleic acid thermodynamics , chemistry , nanotechnology , electrode , nucleotide , organic chemistry , gene , biochemistry , base sequence , chromatography , photocatalysis , catalysis
An electrochemical DNA sensor was constructed using single‐walled carbon nanotubes (SWNTs) attached to a self‐assembled monolayer of 11‐amino‐1‐undecanethiol on a gold surface. The voltammetric peak of methylene blue (MB), which interacts with the DNA guanine bases specifically, was used to follow the DNA hybridization process. After DNA hybridization with its complementary DNA strand, the MB electrochemical signal response decreased and the change in MB signal response was used as the basis for the electrochemical sensing of DNA hybridization. The as described DNA sensor demonstrated to have good stability, selectivity, a linear response over the DNA concentration range from 100 to 1,000 nM and a limit of detection of 7.24 nM.