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A Simple and Effective Way to Integrate Nile Blue Covalently onto Functionalized SWCNTs Modified GC Electrodes for Sensitive and Selective Electroanalysis of NADH
Author(s) -
Saleh Farhana S.,
Rahman Mohammad R.,
Kitamura Fusao,
Okajima Takeyoshi,
Mao Lanqun,
Ohsaka Takeo
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000268
Subject(s) - nile blue , electrode , carbon nanotube , detection limit , overpotential , covalent bond , materials science , chemical engineering , analytical chemistry (journal) , chemistry , nanotechnology , electrochemistry , organic chemistry , chromatography , fluorescence , physics , quantum mechanics , engineering
Abstract A simple and new way to assemble Nile blue (NB) covalently onto the surface of functionalized single‐walled carbon nanotubes (f‐SWCNTs) modified glassy carbon (GC) electrode (NB/f‐SWCNTs/GC electrode) was described. The NB/f‐SWCNTs/GC electrode catalyzes effectively the oxidation of NADH with a remarkably decreased overpotential (ca. 700 mV) compared with that at the bare GC. The reaction was found to obey a so‐called Michaelis–Menten kinetics and the related kinetic parameters were determined. This modified electrode possesses promising characteristics as NADH sensor; a wide linear dynamic range of 0.2 to 200 µM, low detection limit of 0.18 µM, fast response time (1–2 s), high sensitivity (24 µA cm −2 mM −1 ), anti‐interference ability and anti‐fouling.