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Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite
Author(s) -
Wang Wei,
Li HongYi,
Zhang DeWen,
Jiang Jing,
Cui YiRan,
Qiu Shuang,
Zhou YingLin,
Zhang XinXiang
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000235
Subject(s) - glucose oxidase , biosensor , amperometry , nanocomposite , nanofiber , horseradish peroxidase , colloidal gold , cellulose , nanoparticle , bacterial cellulose , detection limit , nuclear chemistry , materials science , chemistry , electrode , chemical engineering , chromatography , nanotechnology , electrochemistry , organic chemistry , enzyme , engineering
An exploration of gold nanoparticles–bacterial cellulose nanofibers (Au‐BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au‐BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au‐BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 µM with a linear range from 10 µM to 400 µM. The biosensor was successfully applied to the determination of glucose in human blood samples.