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The Signal‐Enhanced Label‐Free Immunosensor Based on Assembly of Prussian Blue‐SiO 2 Nanocomposite for Amperometric Measurement of Neuron‐Specific Enolase
Author(s) -
Zhong Zhaoyang,
Shan Jinlu,
Zhang Zhiming,
Qing Yi,
Wang Dong
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000221
Subject(s) - prussian blue , nanocomposite , materials science , amperometry , biocompatibility , detection limit , nanoparticle , monolayer , chemical engineering , nuclear chemistry , biosensor , adsorption , colloidal gold , nanotechnology , electrochemistry , electrode , chemistry , chromatography , organic chemistry , engineering , metallurgy
A signal‐enhanced label‐free electrochemical immunosensor was constructed by the employment of Prussian blue doped silica dioxide (PB‐SiO 2 ) nanocomposite. At first, PB‐SiO 2 nanocomposite which was produced by using a microemulsion method was used to obtain a nanostructural monolayer on a glassy carbon electrode (GCE) surface. Next amino‐functionalized interface were prepared by self‐assembling 3‐aminopropyltriethoxy silane (APTES) on the PB‐SiO 2 nanoparticle surface. Then chitosan stabled gold nanoparticle (CS‐nanoAu) was subsequently attached, while the entire surface was finally loaded with neuron‐specific enolase antibody (anti‐NSE) via the adsorption of gold nanoparticle. The sensitivity of the proposed immunosensor has greatly improved as the PB‐SiO 2 nanostructural sensing film provides plenty of active sites which might catalyze the reduction of H 2 O 2 . The immunosensor exhibited good linear behavior in the concentration range from 0.25–5.0 and 5.0–75 ng/mL for the quantitative analysis of neuron‐specific enolase (NSE), a putative serum marker of small‐cell lung carcinoma (SCLC), with a limit of detection of 0.08 ng/mL. The resulting NSE immunosensor showed high sensitivity and long‐term lifetime which can be attributed to the extremely high catalytic activity and biocompatibility of CS‐nanoAu/APTES/PB‐SiO 2 nanostructural multilayers.